Callus cultures are established from different vegetal tissues explants with the aim to induce a proliferative meristematic-like cell mass. Once calli are stabilized, cell suspension cultures are estabilished.

Further screening of cell cultures, in optimized conditions, is performed by HPLC, LC-MS and NMR analyses in order to characterize the actives profile and to identify the metabolically most efficient cell colonies.

Finally, the scale-up of the cultures from solid culture media, to liquid culture media in flasks, and finally bioreactors allows the achievement of a large volume environment (in the order of M³) where cells grow up and from which the active ingredients are extracted, titrated, analized and finally stored.